Discovery of Potent, Selective Stem Cell Factor Receptor/Platelet Derived Growth Factor Receptor Alpha (c-KIT/PDGFRα) Dual Inhibitor for the Treatment of Imatinib-Resistant Gastrointestinal Stromal Tumors (GISTs)

Yanli Lu; Fei Mao; Xiaokang Li; Xinyu Zheng; Manjiong Wang; Qing Xu; Jin Zhu; Jian Li

doi:10.1021/acs.jmedchem.7b00468

Discovery of Potent, Selective Stem Cell Factor Receptor/Platelet Derived Growth Factor Receptor Alpha (c-KIT/PDGFRα) Dual Inhibitor for the Treatment of Imatinib-Resistant Gastrointestinal Stromal Tumors (GISTs)

J Med Chem. 2017 Jun 22;60(12):5099-5119. doi: 10.1021/acs.jmedchem.7b00468. Epub 2017 Jun 7.

Authors

Yanli Lu¹, Fei Mao¹, Xiaokang Li¹, Xinyu Zheng¹, Manjiong Wang¹, Qing Xu¹, Jin Zhu¹, Jian Li¹

Affiliation

¹ Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology , Shanghai 200237, China.

PMID: 28541695
DOI: 10.1021/acs.jmedchem.7b00468

Abstract

Stem cell factor receptor (c-KIT) and platelet derived growth factor receptor alpha (PDGFRα) kinases play an important role in gastrointestinal stromal tumors (GISTs). Here, we have discovered an c-KIT/PDGFRα dual inhibitor, compound 31, with single-digit nanomolar potency against c-KIT and PDGFRα. Compared to Imatinib (1), 31 showed better antiproliferative efficacy against various TEL-c-KIT/PDGFRα-BaF3 isogenic cells, including three 1-resistant BaF3 cell lines, as well as against GIST-T1 and GIST-882 cell lines. Furthermore, compound 31 showed a good KinomeScan selectivity (468 kinases) (S score (1) = 0.01 at 1 μM concentration), good metabolic stability in liver microsomes, and no hERG inhibitory activity. It was worth noting that 31 inhibited GIST-T1 tumor growth (TGI = 81.5%) and even the BaF3-TEL-cKIT-T670I tumor progression (TGI = 41.9%, 1-resistant GISTs) at a dosage of 100 mg/kg/day without exhibiting apparent toxicity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents / chemistry
Antineoplastic Agents / pharmacology
Cell Line, Tumor
Chemistry Techniques, Synthetic
Drug Resistance, Neoplasm / drug effects*
Drug Screening Assays, Antitumor / methods
Female
Gastrointestinal Stromal Tumors / drug therapy*
Humans
Imatinib Mesylate / pharmacology
Male
Mice, Nude
Molecular Targeted Therapy / methods
Protein Kinase Inhibitors / chemistry
Protein Kinase Inhibitors / pharmacology*
Proto-Oncogene Proteins c-kit / antagonists & inhibitors*
Proto-Oncogene Proteins c-kit / genetics
Proto-Oncogene Proteins c-kit / metabolism
Rats, Sprague-Dawley
Receptor, Platelet-Derived Growth Factor alpha / antagonists & inhibitors*
Receptor, Platelet-Derived Growth Factor alpha / genetics
Receptor, Platelet-Derived Growth Factor alpha / metabolism
Structure-Activity Relationship
Xenograft Model Antitumor Assays

Substances

Antineoplastic Agents
Protein Kinase Inhibitors
Imatinib Mesylate
Proto-Oncogene Proteins c-kit
Receptor, Platelet-Derived Growth Factor alpha